Elimination of the acid fastness but not the gram positivity of leprosy bacilli after extraction with pyridine.
نویسندگان
چکیده
Bacilli from a number of tissues excised from cases of lepromatous and borderline leprosy, unlike mycobacteria, were found to lose their acid-fastness after treatment with pyridine.
منابع مشابه
Cytochemical reactions of human leprosy bacilli and mycobacteria: ultrastructural implications.
Leprosy bacilli harvested from freshly biopsied tissue from cases of lepromatous, borderline and histoid leprosy were, in conjunction with Mycobacterium lepraemurium and representative mycobacteria, examined cytochemically with and without their pyridine-extractable acid-fastness. Unlike the mycobacteria, unextracted leprosy bacilli failed to give a positive response to the periodic acid Schiff...
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As part of a series of investigations at Baba Baghi Leprosarium in lran, 44 long-treated leprosy patients were selected for our study. Samples of early morning sputum were obtained from each patient, examined by microscopy for acid-fast bacilli (AFB), and cultured for tubercle bacilli. These tests were negative, but the polymerase chain reaction (PCR) for an insertion sequence believed to b...
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Introduction: Leprosy is an ancient deforming disease caused by Mycobacterium leprae, which is still poorly understood and often feared by the general public and even by some in the health care professions. Fortunately, the outlook for patients has dramatically improved over the last three decades with the introduction of multi-drug treatment and management strategies that have somewhat dim...
متن کاملProcedure for demonstrating lepra bacilli in paraffin sections.
The lesser degree of acid-fastness of lepra bacilli as compared with tubercle bacilli appears in a disagreeable manner in the difficulty of demonstrating the organisms of leprosy in paraffinized tissues (1). Faraco (2) showed that by ordinary methods of demonstrating acid-fast organisms the lepra bacilli are often not acid-fast, are not differentiated and may be stained by the counterstain. He ...
متن کاملDual Nature of Acid-Fastness **
Acid-fastness is usually defined by the Ziehl-Neelsen technique: staining in carbol-fuchsin, differentiation in HCl-alcohol, and counterstaining in methylene blue.8 As a result, acid-fast substances are red, non-acid-fast materials, blue. Some authors have felt that this property of differential staining resided in one specific type of compound, the mycolic acid type of long-chain fatty acid fo...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 106 2 شماره
صفحات -
تاریخ انتشار 1971